Title

REVOX PA Liquid Sterilant

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Peracetic Acid as a Cold and Non-toxic Liquid Biocide

REVOX sterilization technology uses a room temperature vapor composed of acetic acid and peracetic acid (PAA).

PAA is formed by the reaction of acetic acid and H2O2 with the addition of a catalyst; these compounds exist in equilibrium and their eventual decomposition results in non-toxic oxygen, carbon dioxide and water.

Chemical formulation of peracetic acid for cold sterilization

Mode of Action

The vaporized peracetic acid (VPA) interacts with numerous cellular constituents breaking them down and inactivating routine functionality. With the disintegration of the bacterial cell wall, internal components will no longer be contained and are unable to organize. 

VPA will inactivate gram-positive and gram-negative bacteria, fungi, and yeasts in <5 minutes at <100 ppm. In the presence of organic matter, 200-500 ppm is required. For viruses, the dosage range is wide (12-2,250 ppm), with poliovirus inactivated in yeast extract in 15 minutes with 1,500 to 2,250 ppm. Bacterial spores in suspension are inactivated in 15 seconds to 30 minutes with 500 to 10,000 ppm (0.05 to 1%) 1.

Dr. Michelle Alfa, Clincial Microbiologist, along with her co-workers, compared a VPA system with ethylene oxide (EO) and demonstrated the high efficacy of such a VPA system. Only the VPA system was able to completely kill 6-log10 of Mycobacterium chelonae, Enterococcus faecalis, and B. atrophaeus spores with both an organic and inorganic challenge2. Like other sterilization processes, the efficacy of the process can be diminished by soil challenges and test conditions3.

References

1. Block SS. Peroxygen compounds. In: Block SS, ed. Disinfection, sterilization, and preservation. Philadelphia: Lippincott Williams & Wilkins, 2001:979-96.

2. Alfa MJ, DeGagne P, Olson N, Hizon R. Comparison of liquid chemical sterilization with peracetic acid and ethylene oxide sterilization for long narrow lumens. Am. J. Infect. Control 1998; 26:469-77.

3. 7. Rutala WA, Gergen MF, Weber DJ. Comparative evaluation of the sporicidal activity of new low-temperature sterilization technologies: Ethylene oxide, 2 plasma sterilization systems, and liquid peracetic acid. Am. J. Infect. Control 1998; 26:393-8. Describing Peracetic Acid Vapor Sterilization